Rowe B P, Saylor D L, Speth R C
Department of Physiology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614.
Brain Res. 1990 Nov 26;534(1-2):129-34. doi: 10.1016/0006-8993(90)90122-r.
Angiotensin II (AII) immunoreactivity in the mesopontine area of the rat brain is distributed through several areas where co-localization of AII receptors has not been established. The current in vitro receptor autoradiography study re-examined the distribution of AII binding using 125I-Sar1,Ile8-AII ([125I]SIAII). When incubations were conducted without sulfhydryl reducing agents, [125I]SIAII binding was observed in the locus coeruleus, inferior colliculus, superior colliculus and the central gray in agreement with previous reports. Novel [125I]SIAII binding sites were detected in the parabrachial nucleus, pedunculopontine tegmental nucleus and the caudal linear raphe nucleus, corresponding with previously reported localization of AII immunoreactivity in these nuclei. [125I]SIAII binding was also found in the paragenual nucleus where the peptide has not been detected. Thus, the observation of novel AII receptors which are sensitive to sulfhydryl reducing agents, resolves several AII-AII receptor mismatches.
血管紧张素II（AII）在大鼠脑桥中脑区域的免疫反应性分布于几个尚未确定AII受体共定位的区域。当前的体外受体放射自显影研究使用125I- Sar1，Ile8-AII（[125I] SIAII）重新检查了AII结合的分布。当在没有巯基还原剂的情况下进行孵育时，与先前的报告一致，在蓝斑、下丘、上丘和中央灰质中观察到[125I] SIAII结合。在臂旁核、脚桥被盖核和尾侧中缝核中检测到新的[125I] SIAII结合位点，这与先前报道的这些核中AII免疫反应性的定位一致。在尚未检测到该肽的旁膝核中也发现了[125I] SIAII结合。因此，对巯基还原剂敏感的新型AII受体的观察解决了几个AII-AII受体不匹配的问题。
