Leflon P, Plaquet R, Morinière P, Fournier A
Biochemistry Laboratory, University Hospital, Amiens, France.
Clin Chim Acta. 1990 Oct 31;191(1-2):31-8. doi: 10.1016/0009-8981(90)90055-w.
We propose a new method for measuring aluminium in bone tissue, using argon plasma emission spectrophotometry. The detection limit in the bone nitric digestion liquid was 0.015 mumol/l (corresponding to 0.0075 mumol/g, i.e. 0.2 microgram/g for a tissue sample with 1.0 g wet weight). Within-run CVs were 4.66% and 1.43% for tissues containing 0.15 and 0.64 mumol/g, respectively. Other bone constituents such as calcium, phosphorus, magnesium, sodium, potassium, do not affect aluminum results. Normal values obtained in bone of subjects not suspected of aluminium intoxication were: mean +/- SD; 0.09 +/- 0.044 mumol/g (n = 24). In dialysis patients we found a mean bone aluminium content of 0.75 mumol/g for concentrations ranging between 0.16 and 3.38 mumol/g.
我们提出了一种使用氩等离子体发射分光光度法测量骨组织中铝含量的新方法。骨硝酸消化液中的检测限为0.015微摩尔/升（相当于0.0075微摩尔/克，即对于湿重为1.0克的组织样本为0.2微克/克）。对于分别含有0.15和0.64微摩尔/克的组织，批内变异系数分别为4.66%和1.43%。其他骨成分如钙、磷、镁、钠、钾不影响铝的检测结果。在未怀疑有铝中毒的受试者的骨中获得的正常值为：均值±标准差；0.09±0.044微摩尔/克（n = 24）。在透析患者中，我们发现骨铝含量均值为0.75微摩尔/克，浓度范围在0.16至3.38微摩尔/克之间。
