Ford T C, Graham J, Rickwood D
Department of Cellular and Molecular Sciences, St. Georges Hospital Medical School, London, UK.
Clin Chim Acta. 1990 Nov 30;192(2):115-9. doi: 10.1016/0009-8981(90)90075-4.
We describe a new, rapid method for the isolation of platelets from human blood using a single centrifugation step through Nycodenz, an inert, nontoxic, nonionic medium. As judged by aggregation and nucleotide release studies, the platelets are recovered in high yield and in excellent functional condition.
我们描述了一种新的、快速的从人血中分离血小板的方法,该方法通过在惰性、无毒、非离子介质Nycodenz中进行单次离心步骤来实现。通过聚集和核苷酸释放研究判断,血小板的回收率很高,且功能状态极佳。