Mason R G, Read M S, Shermer R W
Am J Pathol. 1974 Aug;76(2):323-32.
Human platelets separated from blood by six different methods have been compared for aggregability, adhesion to glass, adenine nucleotide content and release, and adenosine triphosphatase and cholinesterase activities. Methods of separation of platelets from blood included three differential centrifugation technics, gel filtration and two albumin density gradient methods. Platelets prepared by the different methods aggregated comparably except those separated by albumin density gradient technics which tended to be hyporeactive. Differences in adhesion to glass, adenine nucleotide content and release, and monitored enzyme activities of the various platelet preparations were noted in several cases but were not marked in general. Ultrastructural studies, reported elsewhere, revealed that platelets separated by the method of Mustard or by gel filtration were less altered morphologically than those separated by the other methods. Platelets separated from blood by gel filtration also appeared somewhat superior functionally to platelets separated by other methods.
通过六种不同方法从血液中分离出的人体血小板，在聚集性、对玻璃的黏附性、腺嘌呤核苷酸含量及释放情况，以及三磷酸腺苷酶和胆碱酯酶活性方面进行了比较。从血液中分离血小板的方法包括三种差速离心技术、凝胶过滤法和两种白蛋白密度梯度法。除了通过白蛋白密度梯度技术分离的血小板往往反应性较低外，用不同方法制备的血小板聚集情况相当。在几种情况下，注意到各种血小板制剂在对玻璃的黏附性、腺嘌呤核苷酸含量及释放情况以及监测到的酶活性方面存在差异，但总体上并不明显。在其他地方报道的超微结构研究表明，通过芥末法或凝胶过滤法分离的血小板在形态上的改变比通过其他方法分离的血小板要小。通过凝胶过滤法从血液中分离出的血小板在功能上似乎也比通过其他方法分离的血小板略胜一筹。
