基于表面增强拉曼光谱的无标记 DNA 杂交检测
Label-free detection of DNA hybridization using surface enhanced Raman spectroscopy.
机构信息
Department of Chemistry, Rice University, Houston, Texas 77005, USA.
出版信息
J Am Chem Soc. 2010 Sep 22;132(37):12792-3. doi: 10.1021/ja105678z.
The SERS spectrum of DNA is strongly dominated by the strong spectral feature of adenine at 736 cm(-1); the presence of adenine can serve as an endogenous marker for the label-free SERS-based detection of DNA hybridization when the probe DNA sequence is adenine-free. The substitution of 2-aminopurine for adenine on the probe DNA sequence enables the detection of a target sequence using SERS, upon hybridization of the target with the 2-AP-substituted probe DNA sequence.
DNA 的 SERS 光谱主要由腺嘌呤在 736cm(-1)处的强光谱特征主导；当探针 DNA 序列不含腺嘌呤时，腺嘌呤的存在可以作为无标记 SERS 基 DNA 杂交检测的内源性标记。在目标与 2-AP 取代的探针 DNA 序列杂交时，用 2-氨基嘌呤取代探针 DNA 序列上的腺嘌呤，可以利用 SERS 检测目标序列。
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