Foodborne Bacterial Pathogens and Mycology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL, USA.
FEMS Microbiol Lett. 2010 Oct;311(2):140-6. doi: 10.1111/j.1574-6968.2010.02082.x. Epub 2010 Aug 25.
Sophorolipids are carbohydrate-based, amphiphilic biosurfactants that are of increasing interest for use in environmentally benign cleaning agents. Sophorolipid production was tested for 26 strains representing 19 species of the Starmerella yeast clade, including Starmerella bombicola and Candida apicola, which were previously reported to produce sophorolipids. Five of the 19 species tested showed significant production of sophorolipids: S. bombicola, C. apicola, Candida riodocensis, Candida stellata and a new species, Candida sp. NRRL Y-27208. A high-throughput matrix-assisted laser desorption/ionization-time of flight MS assay was developed that showed S. bombicola and C. apicola to produce a lactone form of sophorolipid, whereas C. riodocensis, C. stellata and Candida sp. NRRL Y-27208 produced predominantly free acid sophorolipids. Phylogenetic analysis of sequences for the D1/D2 domains of the nuclear large subunit rRNA gene placed all sophorolipid-producing species in the S. bombicola subclade of the Starmerella clade.
槐烷糖苷是一种基于碳水化合物的两亲性生物表面活性剂，由于其在环境友好型清洁剂中的应用而受到越来越多的关注。对代表星美酵母群的 19 个种的 26 株菌进行了槐烷糖苷生产测试，包括先前报道能产生槐烷糖苷的星美棒杆菌和蜂房毕赤酵母。在测试的 19 个种中有 5 个种显示出明显的槐烷糖苷产生：星美棒杆菌、蜂房毕赤酵母、里氏毕赤酵母、星状假丝酵母和一个新种，即 Candida sp. NRRL Y-27208。开发了一种高通量基质辅助激光解吸/电离飞行时间 MS 测定法，表明星美棒杆菌和蜂房毕赤酵母产生槐烷糖苷的内酯形式，而里氏毕赤酵母、星状假丝酵母和 Candida sp. NRRL Y-27208 主要产生游离酸槐烷糖苷。核大亚基 rRNA 基因的 D1/D2 结构域的序列系统发育分析将所有产生槐烷糖苷的种都置于星美棒杆菌亚群中。
