Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Ghent University , K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.
J Proteome Res. 2013 Oct 4;12(10):4376-92. doi: 10.1021/pr400392a. Epub 2013 Sep 11.
Starmerella (Candida) bombicola is the biosurfactant-producing species that caught the greatest deal of attention in the academic and industrial world due to its ability of producing large amounts of sophorolipids. Despite its high economic potential, the biochemistry behind the sophorolipid biosynthesis is still poorly understood. Here we present the first proteomic characterization of S. bombicola for which we created a lys1Δ mutant to allow the use of SILAC for quantitative analysis. To characterize the processes behind the production of these biosurfactants, we compared the proteome of sophorolipid producing (early stationary phase) and nonproducing cells (exponential phase). We report the simultaneous production of all known enzymes involved in sophorolipid biosynthesis including a predicted sophorolipid transporter. In addition, we identified the heme binding protein Dap1 as a possible regulator for Cyp52M1. Our results further indicate that ammonium and phosphate limitation are not the sole limiting factors inducing sophorolipid biosynthesis.
假丝酵母（ Candida ）炸弹菌是一种能够产生大量槐糖脂的生物表面活性剂产生菌，因此在学术界和工业界引起了广泛关注。尽管它具有很高的经济潜力，但槐糖脂生物合成的生物化学仍知之甚少。在这里，我们首次对假丝酵母（ Candida ）炸弹菌进行了蛋白质组学表征，为此我们创建了 lys1Δ 突变体，以允许使用 SILAC 进行定量分析。为了表征这些生物表面活性剂生产背后的过程，我们比较了产生生物表面活性剂的（早期静止期）和不产生生物表面活性剂的细胞（指数生长期）的蛋白质组。我们报告了所有已知参与槐糖脂生物合成的酶的同时生产，包括预测的槐糖脂转运蛋白。此外，我们还确定了血红素结合蛋白 Dap1 作为 Cyp52M1 的可能调节剂。我们的研究结果进一步表明，铵和磷酸盐的限制不是唯一诱导槐糖脂生物合成的限制因素。
