Claas H C, Melchers W J, de Bruijn I H, de Graaf M, van Dijk W C, Lindeman J, Quint W G
Department of Molecular Biology, Diagnostic Centre SSDZ, Delft, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1990 Dec;9(12):864-8. doi: 10.1007/BF01967500.
Sequences derived from the endogenous plasmid of Chlamydia trachomatis and from the genes coding for ribosomal 16S RNA of Chlamydia psittaci were used as primers and oligonucleotide probes for detection of chlamydiae by the polymerase chain reaction. The endogenous plasmid primers generated specific amplified products of 517 bp with all known Chlamydia trachomatis serovars. No specific products of Chlamydia psittaci and Chlamydia pneumoniae could be detected using these primers. With the rRNA primers specific amplified products of 208 bp were generated with Chlamydia psittaci, Chlamydia trachomatis and Chlamydia pneumoniae. No specific amplified products were detected with DNA isolated from a variety of microorganisms from the urogenital and the respiratory tract. Of 156 clinical specimens used for evaluation of the polymerase chain reaction, 26 were found to be positive for Chlamydia trachomatis on culture. All 26 culture positive samples were also found to be positive for Chlamydia trachomatis DNA by the polymerase chain reaction with both primer sets. Two culture negative samples were also found to be positive by this technique. The polymerase chain reaction thus seems to be a sensitive and reliable method for detection of Chlamydia trachomatis.
沙眼衣原体的内源性质粒衍生序列和鹦鹉热衣原体核糖体16S RNA编码基因的序列被用作引物和寡核苷酸探针,通过聚合酶链反应检测衣原体。内源性质粒引物与所有已知的沙眼衣原体血清型产生了517 bp的特异性扩增产物。使用这些引物未检测到鹦鹉热衣原体和肺炎衣原体的特异性产物。rRNA引物与鹦鹉热衣原体、沙眼衣原体和肺炎衣原体产生了208 bp的特异性扩增产物。从泌尿生殖道和呼吸道的多种微生物中分离的DNA未检测到特异性扩增产物。在用于评估聚合酶链反应的156份临床标本中,26份经培养发现沙眼衣原体呈阳性。通过聚合酶链反应,使用两组引物,所有26份培养阳性样本的沙眼衣原体DNA也呈阳性。另外两份培养阴性样本通过该技术也呈阳性。因此,聚合酶链反应似乎是一种检测沙眼衣原体的灵敏且可靠的方法。