School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, PR China.
Phytomedicine. 2011 Feb 15;18(4):292-7. doi: 10.1016/j.phymed.2010.07.005. Epub 2010 Aug 23.
Belamcanda chinensis (Iridaceae) belongs to the family of iridaceae and its rhizoma has been widely used for the treatment of throat ailment. Here we report a new pharmacological activity of B. chinensis leaf extract (BCL), that is, the hypoglycemic effect in normal and STZ-induced diabetic rats. Animals either healthy or STZ-induced diabetic show significantly lowered fasting blood glucose levels after treatment with BCL. The serum insulin concentration in normal rats is also enhanced. Additionally, the increase in blood glucose levels after administration of various carbohydrates in normal rats is significantly decreased and the oral glucose tolerance (OGTT) of STZ-induced diabetic rats is largely improved by BCL treatment. However, co-administration of BCL with Nifedipine, a Ca(2+) ion channel blocker, or Nicorandil, an ATP-sensitive K(+) ion channel opener thoroughly abolishes the hypoglycemic effect of BCL. HPLC analysis and compound identification showed that several isoflavone glycosides with antidiabetic activities were contained in BCL while pharmacological experiment showed that the polysaccharide fraction of BCL had no significant hypoglycemic effect on normal rats. Therefore, the isoflavone glycosides but not polysaccharides might be the active fraction of BCL in diabetes treatment.
射干（鸢尾科）属于鸢尾科，其根茎被广泛用于治疗咽喉疾病。在这里，我们报告了射干叶提取物（BCL）的一种新的药理活性，即对正常和 STZ 诱导的糖尿病大鼠的降血糖作用。无论是健康动物还是 STZ 诱导的糖尿病动物，用 BCL 治疗后空腹血糖水平均显著降低。正常大鼠的血清胰岛素浓度也得到增强。此外，BCL 处理可显著降低正常大鼠给予各种碳水化合物后血糖水平的升高，显著改善 STZ 诱导的糖尿病大鼠的口服糖耐量（OGTT）。然而，BCL 与硝苯地平（一种 Ca(2+) 离子通道阻滞剂）或尼可地尔（一种 ATP 敏感性 K(+) 离子通道开放剂）联合给药会彻底消除 BCL 的降血糖作用。HPLC 分析和化合物鉴定表明，BCL 中含有几种具有降血糖活性的异黄酮糖苷，而药理实验表明 BCL 的多糖部分对正常大鼠没有显著的降血糖作用。因此，异黄酮糖苷而不是多糖可能是 BCL 在糖尿病治疗中的有效成分。
