Talba Mahamad Abba, Miaffo David, Poualeu Kamani Sylviane Laure, Kamanyi Albert, Wansi Sylvie Léa
Department of Biological Sciences, Faculty of Sciences, University of Maroua, Far North Region, Maroua, Cameroon.
Department of Life and Earth Sciences, Higher Teachers' Training College, University of Maroua, Far North Region, Maroua, Cameroon.
Evid Based Complement Alternat Med. 2019 Nov 16;2019:7384532. doi: 10.1155/2019/7384532. eCollection 2019.
(Vitaceae) is a plant used in Cameroonian traditional medicine for the treatment of diabetes. The aims of this study were to evaluate the in vitro antioxidant and antidiabetic activities of the aqueous extract of leafy stems. The enzyme inhibitory activity was determined in vitro on -amylase and -glucosidase enzymes, followed by confirmative study in vivo on normal rats (oral starch and sucrose tolerance tests at doses of 111, 222, and 444 mg/kg). The ferric reducing antioxidant power and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antiradical activity of the extract were examined to evaluate the antioxidant potential of the extract. The total content of phenols, flavonoids, and tannins of the extract were also determined. The results showed an inhibitory effect of the extract on the -amylase and -glucosidase activities with IC values of 216.14 and 182.40 g/mL, respectively. The extract at doses of 222 and 444 mg/kg induced a significant decrease in postprandial glycaemia during the starch and sucrose tolerance tests. A remarkable antiradical activity of the extract was obtained although lower than that of the standard product. The aqueous extract of leafy stems of has an interesting inhibitory activity on the -amylase and -glucosidase enzymes, as well as an antioxidant potential, thus validating its use in traditional medicine for the treatment of diabetes mellitus and its complications.
（葡萄科）是一种用于喀麦隆传统医学治疗糖尿病的植物。本研究的目的是评估叶状茎水提取物的体外抗氧化和抗糖尿病活性。体外测定提取物对α-淀粉酶和α-葡萄糖苷酶的酶抑制活性，随后在正常大鼠体内进行验证性研究（分别以111、222和444mg/kg的剂量进行口服淀粉和蔗糖耐量试验）。检测提取物的铁还原抗氧化能力和2,2-二苯基-1-苦基肼（DPPH）抗自由基活性，以评估提取物的抗氧化潜力。还测定了提取物中酚类、黄酮类和单宁的总含量。结果表明，提取物对α-淀粉酶和α-葡萄糖苷酶活性有抑制作用，IC值分别为216.14和182.40μg/mL。在淀粉和蔗糖耐量试验中，222和444mg/kg剂量的提取物可显著降低餐后血糖。提取物具有显著的抗自由基活性，尽管低于标准产品。叶状茎的水提取物对α-淀粉酶和α-葡萄糖苷酶具有有趣的抑制活性，以及抗氧化潜力，从而验证了其在传统医学中用于治疗糖尿病及其并发症的用途。
