Sakanian V A, Ovespian A S, Mett I L, Kochikian A V, Petrosian P K
Genetika. 1990 Nov;26(11):1915-25.
Genes encoding arginine biosynthesis of Bacillus stearothermophilus strain 718 were cloned in the mutant argA strain of the Escherichia coli K-12. The arg genes were shown to be located on the 3.7 kb DNA fragment in the following order: argA--argE--argB. The expression of the argA gene of B. stearothermophilus on the multicopy vehicle is twofold higher in argR- strain of E. coli K-12 than is isogenic argR+ strain. According to hybridization analysis argA genes of B. stearothermophilus and B. subtilis have low level of homology, which is the evidence of their evolutional divergence.
嗜热脂肪芽孢杆菌718株编码精氨酸生物合成的基因被克隆到大肠杆菌K-12的突变型argA菌株中。这些精氨酸基因被证明位于3.7 kb的DNA片段上，顺序如下：argA--argE--argB。嗜热脂肪芽孢杆菌的argA基因在多拷贝载体上，在大肠杆菌K-12的argR-菌株中的表达比同基因的argR+菌株高两倍。根据杂交分析，嗜热脂肪芽孢杆菌和枯草芽孢杆菌的argA基因具有低水平的同源性，这证明了它们在进化上的差异。
