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[携带大肠杆菌K-12精氨酸生物合成基因的重组质粒的限制酶切图谱分析]

[Restriction mapping of recombinant plasmids carrying the genes for arginine biosynthesis in Escherichia coli K-12].

作者信息

Nersisian A A, Mett I L, Badalian Z R, Kochikian A V, Mett A L

出版信息

Genetika. 1986 Jul;22(7):1061-6.

PMID:3017809
Abstract

ArgA and argECBH genes of Escherichia coli K-12 were cloned on the pBR322 vector. Restriction maps of the recombinant plasmids were constructed. Deletion mutants of these recombinant plasmids, retaining the functional argA and argE genes, were obtained using different restriction enzymes. All of the recombinant derivatives have the replication properties of the pBR322 vector.

摘要

将大肠杆菌K-12的ArgA和argECBH基因克隆到pBR322载体上。构建了重组质粒的限制性图谱。使用不同的限制性内切酶获得了保留功能性argA和argE基因的这些重组质粒的缺失突变体。所有重组衍生物都具有pBR322载体的复制特性。

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