Institute of Pathology, Klinikum Augsburg, Augsburg, Germany.
J Surg Oncol. 2010 Sep 1;102(3):235-41. doi: 10.1002/jso.21611.
The proteases PAI-1 and uPA play a major role in extracellular matrix degradation, which facilitates tumour progression. Tumour budding is a histomorphological expression of enhanced tumour cell migration.
To investigate their prognostic value for and correlation with colon cancer, a prospective study was performed. We analysed tissue levels of uPA and PAI-1 of 55 colon cancer tumours employing a commercially available enzyme-linked immunosorbent assay (ELISA). Tumour budding was analysed on cytokeratin-stained slides.
There was a strong correlation between uPA and tumour budding (R = 0.440; P < 0.001). uPA levels were increased in high grade tumours, whereas PAI-1 was elevated in cases with venous invasion (P = 0.004 and P = 0.028). PAI-1 values and tumour budding are associated significantly with the occurrence of distant metastases (P < 0.001 and P = 0.034, respectively). Tumour budding was significantly associated with lymph node metastases (P = 0.034). Multivariate analysis revealed PAI-1 and lymph node metastases to be independently predictive of distant metastases (P = 0.007 and P = 0.004, respectively).
The results of our study show that tumour budding and the plasmin/plasminogen system are related. PAI-1 was independently predictive for the occurrence of distant metastasis.
蛋白酶 PAI-1 和 uPA 在细胞外基质降解中起主要作用，从而促进肿瘤进展。肿瘤出芽是肿瘤细胞迁移增强的组织形态学表现。
为了研究其对结肠癌的预后价值及其与结肠癌的相关性，进行了一项前瞻性研究。我们采用商业上可用的酶联免疫吸附测定（ELISA）分析了 55 例结肠癌肿瘤的 uPA 和 PAI-1 组织水平。在细胞角蛋白染色的幻灯片上分析肿瘤出芽。
uPA 与肿瘤出芽之间存在很强的相关性（R = 0.440；P <0.001）。高分级肿瘤中 uPA 水平升高，而静脉侵犯时 PAI-1 升高（P = 0.004 和 P = 0.028）。PAI-1 值和肿瘤出芽与远处转移的发生显著相关（P <0.001 和 P = 0.034）。肿瘤出芽与淋巴结转移显著相关（P = 0.034）。多变量分析显示 PAI-1 和淋巴结转移是远处转移的独立预测因素（P = 0.007 和 P = 0.004）。
我们的研究结果表明，肿瘤出芽和纤溶酶/纤溶酶原系统有关。PAI-1 是远处转移发生的独立预测因子。
