Cho-Chung Y S, Bodwin J S, Clair T
Eur J Biochem. 1978 May;86(1):51-60. doi: 10.1111/j.1432-1033.1978.tb12283.x.
Dimethylbenzanthracene-induced rat carcinomas possess activities binding cyclic adenosine 3':5'-monophosphate (cAMP) and estrogen. When dimethylbenzanthracene-induced tumors regress after ovariectomy of the host, a change in the specific binding of cAMP and estrogen occurs in the tumors. Six days after ovariectomy, cAMP binding increases 5-fold in the nuclei and 2-fold in the cytosol of tumors, while nuclear and cytoplasmic estrogen binding decreases by 80% and 50%, respectively. These changes in activities binding cAMP and estrogen are detectable within 1 day after ovariectomy and the changes are reversed when resumption of tumor growth is induced by the injection of 17beta-estradiol. When dimethylbenzanthracene-induced tumors fail to regress after ovariectomy, the change in activities binding cAMP and estrogen does not occur. Significant increases in the cAMP level as well as in adenylate cyclase and cAMP-phosphodiesterase activities are also found in the regressing tumors. Concomitant with the increase of cAMP-binding activity is an increase in histone kinase activity in the regressing tumor. These data suggest the involvement of cAMP in the growth control of a hormone-dependent mammary rumor.
二甲基苯并蒽诱导的大鼠癌具有结合环磷酸腺苷（cAMP）和雌激素的活性。当宿主卵巢切除后二甲基苯并蒽诱导的肿瘤消退时，肿瘤中cAMP和雌激素的特异性结合会发生变化。卵巢切除术后6天，肿瘤细胞核中cAMP结合增加5倍，细胞质中增加2倍，而细胞核和细胞质中的雌激素结合分别减少80%和50%。卵巢切除术后1天内即可检测到cAMP和雌激素结合活性的这些变化，当通过注射17β-雌二醇诱导肿瘤生长恢复时，这些变化会逆转。当卵巢切除后二甲基苯并蒽诱导的肿瘤未消退时，cAMP和雌激素结合活性不会发生变化。在消退的肿瘤中还发现cAMP水平以及腺苷酸环化酶和cAMP磷酸二酯酶活性显著增加。与cAMP结合活性增加相伴的是消退肿瘤中组蛋白激酶活性增加。这些数据表明cAMP参与了激素依赖性乳腺肿瘤的生长控制。
