Miller W R, Senbanjo R O, Telford J, Watson D M
Br J Cancer. 1985 Oct;52(4):531-5. doi: 10.1038/bjc.1985.224.
The characteristics of a method for measuring cyclic AMP binding proteins in cytosols of human breast cancer are described. Using the assay, binding proteins were demonstrable in all of 100 tumour cytosols. Levels of binding in individual tumours varied from 0.8 to 15 pmol mg-1 cytosol protein (mean value 5 pmol mg-1 cytosol protein) and the dissociation constant ranged from 0.5 to 5.2 X 10(-8)M (mean 1.73 X 10(-8)M). Whilst replicate measurements within a single portion of tumour were reproducible (intra-assay coefficient of variation was between 4.5 and 7.8% and that for inter-assay variation was between 2.1 and 4.0%) there were often considerable differences in levels of binding proteins between different portions of the same tumour. Similar intra-tumour variations have been reported for other binding proteins and steroid receptors. The inter-relationships with such parameters may elucidate whether the differences are associated with variations in cellularity, cell type, or other specific factors.
本文描述了一种测量人乳腺癌细胞溶质中环磷酸腺苷（cAMP）结合蛋白的方法的特点。使用该检测方法，在100个肿瘤细胞溶质中均能检测到结合蛋白。各个肿瘤中结合蛋白的水平在0.8至15皮摩尔/毫克细胞溶质蛋白之间变化（平均值为5皮摩尔/毫克细胞溶质蛋白），解离常数在0.5至5.2×10⁻⁸摩尔/升之间（平均值为1.73×10⁻⁸摩尔/升）。虽然在肿瘤的单个样本内重复测量具有可重复性（批内变异系数在4.5%至7.8%之间，批间变异系数在2.1%至4.0%之间），但同一肿瘤的不同部分之间结合蛋白水平往往存在显著差异。对于其他结合蛋白和类固醇受体，也报道了类似的肿瘤内变异情况。与这些参数的相互关系可能有助于阐明这些差异是否与细胞数量、细胞类型或其他特定因素的变化有关。
