来自粟酒裂殖酵母的细胞色素c。1. 纯化、光谱性质及氨基酸组成。
Cytochrome c from Schizosaccharomyces pombe. 1. Purification, spectral properties, and amino-acid composition.
作者信息
Claisse M L, Simon-Becam A M
出版信息
Eur J Biochem. 1978 May 16;86(2):399-406. doi: 10.1111/j.1432-1033.1978.tb12322.x.
Cytochrome c from the fission yeast Schizosaccharomyces pombe has been purified. Its chromatographic and spectral properties are reported and compared to those of iso-1-cytochrome c from baker's yeast; the amino-acid composition is described. Schiz. pombe cytochrome c has a much lower affinity for Amberlite IRP64 than Sacch. cerevisiae iso-1-cytochrome c. Its alpha absorption band splits into three maxima (calpha1, calpha2, and calpha3) at -190 degrees C; this is unusual in yeasts, as shown by the low-temperature whole-cell absorption spectra which were examined in various yeast genera, species, and strains. A minor component can be separated by Amberlite chromatography. It exhibits the same low-temperature splitting of the alpha absorption band as the main fraction and it has a similar amino-acid composition with a notable exception: it is an unmethylated form of the cytochrome.
来自裂殖酵母粟酒裂殖酵母的细胞色素c已被纯化。报告了其色谱和光谱特性，并与来自面包酵母的同工酶-1-细胞色素c的特性进行了比较；描述了其氨基酸组成。粟酒裂殖酵母细胞色素c对Amberlite IRP64的亲和力远低于酿酒酵母同工酶-1-细胞色素c。其α吸收带在-190℃时分裂为三个最大值（cα1、cα2和cα3）；这在酵母中是不寻常的，如在各种酵母属、种和菌株中检测到的低温全细胞吸收光谱所示。一个次要成分可以通过Amberlite色谱法分离。它与主要部分一样，在低温下α吸收带会分裂，并且具有相似的氨基酸组成，但有一个显著例外：它是细胞色素的未甲基化形式。
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