Shih H H, Chen S N
Department of Zoology, National Taiwan University, Taipei, ROC.
Southeast Asian J Trop Med Public Health. 1990 Sep;21(3):475-81.
Juvenile worms of Angiostrongylus cantonensis recovered from subarachnoid spaces and pulmonary arteries of rats, respectively, at 28 days post-infection have been compared with respect to their surface composition, antigenicity of surface proteins and morphological appearance. Quantitative and qualitative differences were shown between surface proteins of these two stages of worms. One major and 6 minor proteins appeared on brain stage worm's surface as assessed by surface-labelling and SDS-PAGE techniques. The same, but more predominant banding pattern, with one additional major protein of Mr 80,000 kDa presented on the lung stage worm's surface. Surface components from both stages were antigenic in permissive rat hosts but refractory in nonpermissive human hosts. The surface antigens are common to both stages within the rat. Observed by scanning electron microscopy, the surface appearance of brain stage worms is thickened, rough and irregular. Besides, particle clusters adhere randomly, without cluster adherence but transverse and longitudinal clefts were shown on the surface, before the outer layer was shed. The possible mechanisms of evasion from the host's immune attack with the surface-shedding phenomenon remain to be elucidated.
分别在感染后28天从大鼠蛛网膜下腔和肺动脉中回收的广州管圆线虫幼虫，就其表面组成、表面蛋白的抗原性和形态外观进行了比较。这两个阶段的蠕虫表面蛋白存在数量和质量上的差异。通过表面标记和SDS-PAGE技术评估，脑期蠕虫表面出现一种主要蛋白和6种次要蛋白。肺期蠕虫表面呈现相同但更为主导的条带模式，还有一种额外的80,000 kDa主要蛋白。两个阶段的表面成分在易感大鼠宿主中具有抗原性，但在不易感的人类宿主中无抗原性。大鼠体内这两个阶段的表面抗原是共有的。通过扫描电子显微镜观察，脑期蠕虫的表面外观增厚、粗糙且不规则。此外，颗粒簇随机附着，没有簇附着，但在表层脱落之前，表面出现横向和纵向裂缝。表面脱落现象逃避宿主免疫攻击的可能机制仍有待阐明。
