利用致倦库蚊辐照胚胎检测新大陆螺旋蝇。 （注：原文中“Chrysomya bezziana”有误，正确的是“Cochliomyia hominivorax”，中文名新大陆螺旋蝇，这里按纠正后的内容翻译）
The use of irradiated embryos of Chrysomya bezziana for the detection of the Old World screw-worm fly.
作者信息
Clarke G M
机构信息
DPIE/CSIRO Screw-Worm Fly Unit, Boroko, Papua New Guinea.
出版信息
Aust Vet J. 1990 Dec;67(12):452-3. doi: 10.1111/j.1751-0813.1990.tb03062.x.
Irradiation of Chrysomya bezziana embryos 1 h before hatching with doses less than or equal to 7 kilorad (kr) had a significant effect on percentage egg hatch, weights and survival of larvae. Doses greater than or equal to 1 kr allowed larval development to the end of the 3rd instar stage in vitro, but prevented normal pupal development. Cattle with wounds infested with 1st instar larvae derived from irradiated embryos had 3rd instar larvae present after 3 d but these failed to pupate. Thus it would be feasible to use such larvae for wound infestation for the enhanced detection of screw-worm fly in areas where the release of fertile flies is undesirable.
在孵化前1小时用小于或等于7千拉德（kr）的剂量照射致倦库蚊胚胎，对卵孵化率、幼虫体重和存活率有显著影响。大于或等于1 kr的剂量可使幼虫在体外发育至三龄末期，但阻止了正常的蛹发育。伤口被来自经辐照胚胎的一龄幼虫感染的牛，3天后出现了三龄幼虫，但这些幼虫未能化蛹。因此，在不适合释放可育苍蝇的地区，使用这些幼虫进行伤口感染以增强对螺旋锥蝇的检测是可行的。
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