Wikstrand C J, Proctor J O, Hartsell S C, Haughton G
Int J Cancer. 1978 May 15;21(5):639-47. doi: 10.1002/ijc.2910210515.
The standard one-stage lymphocyte adherence inhibition (LAI) assay was used to investigate cellular and humoral immune responses within the murine Rous sarcoma system. Significant specific cellular reactivity to Rous sarcoma antigen extracts was detected when the responder peritoneal exudate cells (PEC) were obtained from mice bearing or immunized against primary or transplanted Rous sarcomas; no cellular reactivity to control methylcholanthrene (MC)-induced tumor antigen extracts was observed. Similarly, specific cell-mediated recognition of MC tumor antigen extract was demonstrated. Initial experiments designed to assess the role of serum components in the LAI assay demonstrated that normal mouse serum of C57BL/10ScSn, B10.D2, or A/WySn origin, either fresh or frozen, obtained from old (over 7 months) or young (under 6 weeks) mice non-specifically abrogated the specific loss of PEC adherence.
采用标准的一步法淋巴细胞黏附抑制（LAI）试验，研究小鼠劳氏肉瘤系统中的细胞免疫和体液免疫反应。当反应细胞腹膜渗出细胞（PEC）取自患有原发性或移植性劳氏肉瘤或经其免疫的小鼠时，检测到对劳氏肉瘤抗原提取物有显著的特异性细胞反应性；未观察到对对照甲基胆蒽（MC）诱导的肿瘤抗原提取物的细胞反应性。同样，也证明了对MC肿瘤抗原提取物的特异性细胞介导识别。旨在评估血清成分在LAI试验中作用的初步实验表明，来自C57BL/10ScSn、B10.D2或A/WySn品系的正常小鼠血清，无论是新鲜的还是冷冻的，取自老年（超过7个月）或幼年（6周以下）小鼠，均非特异性地消除了PEC黏附的特异性丧失。
