Cell-mediated immune reactions directed against syngeneic methylcholanthrene-induced mouse sarcomas: low reproducibility of the results obtained with the tube modification of leukocyte adherence inhibition assay.

The value of the tube modification of leukocyte adherence inhibition (LAI) assay for assessment of cell-mediated reactions directed against tumor-associated antigens of transplantable MC-induced murine sarcomas was examined. It was established that the adherence of leukocytes isolated from spleens of tumor-bearing mice is specifically inhibited with 3 M KCl extracts of corresponding tumor cells. However, the reproducibility of the results obtained with the tube modification of LAI assay was found to be rather low. Using the same experimental protocol and the same 3 M KCl extracts of the sarcoma and control tissues, significant differences between adherence in tumor specific and control samples were only found in approximately 50% of the experiments.