高效液相色谱法测定大鼠脑和脑脊液中的环丙沙星。

High-performance liquid chromatographic determination of ciprofloxacin in rat brain and cerebrospinal fluid.

作者信息

Katagiri Y, Naora K, Ichikawa N, Hayashibara M, Iwamoto K

机构信息

Department of Pharmacy, Shimane Medical University Hospital, Japan.

出版信息

Chem Pharm Bull (Tokyo). 1990 Oct;38(10):2884-6. doi: 10.1248/cpb.38.2884.

DOI:10.1248/cpb.38.2884

PMID:2076578

Abstract

A novel high-performance liquid chromatographic method for the fluorometric determination of a newer quinolone, ciprofloxacin (CPFX), in rat brain and cerebrospinal fluid (CSF) was developed. CPFX in brain homogenate was extracted and injected onto a reversed-phase column without fluorescence derivatization. CSF was directly analyzed without the extraction procedure. Calibration curves were linear over the concentration ranges of 10 to 500 ng/g for brain and 5 to 500 ng/ml for CSF. The recoveries of CPFX added to brain were more than 97% with a coefficient of variation of less than 4%. The present method was sensitive and reliable enough to be utilized for detailed pharmacokinetic studies of CPFX in rat brain and CSF.

摘要

建立了一种新型高效液相色谱法，用于荧光测定大鼠脑和脑脊液（CSF）中一种新型喹诺酮类药物环丙沙星（CPFX）。脑匀浆中的CPFX无需荧光衍生化即可提取并注入反相柱。脑脊液无需提取过程即可直接分析。脑的校准曲线在10至500 ng/g浓度范围内呈线性，脑脊液的校准曲线在5至500 ng/ml浓度范围内呈线性。添加到脑中的CPFX回收率超过97%，变异系数小于4%。本方法灵敏可靠，可用于CPFX在大鼠脑和脑脊液中的详细药代动力学研究。