Schwarz S, Cardoso M, Blobel H
Institut für Bakteriologie und Immunologie der Justus-Liebig-Universität Giessen.
Dtsch Tierarztl Wochenschr. 1990 Nov;97(11):498, 501-3.
A total of 33 Staphylococcus hyicus-cultures from piglets with exudative epidermatitis were analyzed for the presence of antibiotic resistance plasmids. Four small plasmids encoding resistances to chloramphenicol, macrolide-lincosamide-antibiotics, streptomycin or tetracyclines could be identified in plasmid-curing and plasmid-transformation experiments. For further characterization these plasmids were digested with restriction endonucleases. This led to the construction of a specific restriction map for each of the 4 plasmids. On the basis of their restriction maps, these 4 antibiotic resistance plasmids from "porcine" S. hyicus-cultures were compared with the respective resistance plasmids of other staphylococcal species from infections of humans and animals.
对来自患有渗出性皮炎仔猪的33株猪葡萄球菌培养物进行了分析,以检测其是否存在抗生素抗性质粒。在质粒消除和质粒转化实验中,可鉴定出4种编码对氯霉素、大环内酯 - 林可酰胺类抗生素、链霉素或四环素耐药性的小质粒。为了进一步表征,用限制性内切酶对这些质粒进行消化。这为4种质粒中的每一种构建了特定的限制性图谱。根据其限制性图谱,将来自“猪源”猪葡萄球菌培养物的这4种抗生素抗性质粒与来自人和动物感染的其他葡萄球菌物种的相应抗性质粒进行了比较。