Sekiya S, Tanaka N, Takamizawa H
Department of Obstetrics and Gynecology, Chiba University School of Medicine, Japan.
Arch Gynecol Obstet. 1990;248(2):103-10. doi: 10.1007/BF02389582.
Some characteristics of cell biology and the production of various tumor markers were examined using 8 human ovarian cancer cell lines of epithelial origin. Structural abnormalities of chromosomes 1, 3, and 6 were relatively common karyotypic changes among the cell lines. Cytoplasmic estradiol or progesterone receptor was not detected in any of the cell lines. A significant heterogeneity of the production of various tumor markers (ferritin, tissue peptide antigen, carcinoembryonic antigen, carbohydrate antigens 125, and 19-9) was noted among the cell lines grown in culture medium supplemented with serum. Three of the 8 cell lines were adapted to proliferate in completely synthetic serum-free culture medium. In addition to marker substances described above, small amounts of progesterone or human chorionic gonadotropin were produced in 2 of the 3 cell lines grown in serum-free culture medium. These results indicate that various marker substances including tumor markers are not produced consistently by human ovarian cancer cells of epithelial origin.
利用8种上皮来源的人卵巢癌细胞系，研究了细胞生物学的一些特征以及各种肿瘤标志物的产生情况。1号、3号和6号染色体的结构异常是这些细胞系中相对常见的核型变化。在任何一个细胞系中均未检测到细胞质雌二醇或孕酮受体。在添加血清的培养基中培养的细胞系中，各种肿瘤标志物（铁蛋白、组织多肽抗原、癌胚抗原、糖类抗原125和19-9）的产生存在显著异质性。8个细胞系中的3个适应于在完全合成的无血清培养基中增殖。除上述标志物物质外，在无血清培养基中培养的3个细胞系中的2个产生了少量孕酮或人绒毛膜促性腺激素。这些结果表明，包括肿瘤标志物在内的各种标志物物质并非由上皮来源的人卵巢癌细胞一致产生。
