Ishiwata I, Ishiwata C, Kobayashi N, Ishikawa H
Nihon Sanka Fujinka Gakkai Zasshi. 1987 May;39(5):831-6.
We have recently established a cell line, HUOA, of human ovarian serous cystadenocarcinoma. The cultured cells are various shapes; cylindrical, spindle-shaped, roundish, or polygonal. They are rich in cytoplasm and PAS-positive. They proliferate to form multi-layers without contact inhibition, and they easily form mirror ball-like cysts. These cysts free themselves to float in the culture medium. Cell growth is comparatively slow, and the population doubling time is about 80 hours. The plating efficiency is 16%. The cell line has been cultured for 5 years with more than 100 successful passages and is still proliferating. The HUOA line can be transplanted subcutaneously into nude mice and forms a serous cystadenocarcinoma similar to the original tumor, but when transplanted into the intraperitoneal cavity, the HUOA line forms floating cysts similar to those in vitro. We regard this formation of cyst as an expression of differentiation of the cystadenocarcinoma cells.
我们最近建立了一种人卵巢浆液性囊腺癌的细胞系HUOA。培养的细胞形态各异，呈柱状、纺锤形、圆形或多边形。它们富含细胞质且PAS阳性。它们增殖形成多层，无接触抑制，并且容易形成镜球状囊肿。这些囊肿自行漂浮在培养基中。细胞生长相对缓慢，群体倍增时间约为80小时。接种效率为16%。该细胞系已培养5年，成功传代100多次，仍在增殖。HUOA细胞系可皮下移植到裸鼠体内，形成与原发肿瘤相似的浆液性囊腺癌，但当移植到腹腔内时，HUOA细胞系形成与体外相似的漂浮囊肿。我们认为这种囊肿的形成是囊腺癌细胞分化的一种表现。
