Kundu P N, Das A
J Appl Bacteriol. 1985 Jul;59(1):1-5. doi: 10.1111/j.1365-2672.1985.tb01767.x.
A strong calcium gluconate-producing strain of Aspergillus niger (MN181) was obtained by way of mutagenic treatment. Its growth was very slow with moderate sporulation. The strain was treated with N-methyl-N'nitro-N-nitrosoguanidine (MNNG) and some auxotrophic mutants were obtained. All were less productive than the parent strain in producing calcium gluconate. The reduced yield was corrected in the heterokaryons and diploids derived by crossing sister strains. One diploid strain (D4), heterozygous for auxotrophy and conidial colour markers was grown in the presence of 4% alcohol and 31 segregants were isolated which included both haploid and diploid strains. Their yields were studied and some recombinants were obtained which, in spite of the same yield of MN181, showed improvement in giving fast growth and abundant sporulation.
通过诱变处理获得了一株产葡萄糖酸钙能力强的黑曲霉（MN181）。其生长非常缓慢，产孢适度。用N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理该菌株，获得了一些营养缺陷型突变体。所有这些突变体在生产葡萄糖酸钙方面的能力均低于亲本菌株。通过姊妹菌株杂交获得的异核体和二倍体中，产量降低的情况得到了纠正。一个对营养缺陷和分生孢子颜色标记杂合的二倍体菌株（D4）在4%酒精存在的条件下生长，并分离出31个分离子，其中包括单倍体和二倍体菌株。对它们的产量进行了研究，获得了一些重组体，这些重组体尽管产量与MN181相同，但在生长速度和产孢量方面有了改善。
