Rowlands D J, Harris T J, Brown F
J Virol. 1978 May;26(2):335-43. doi: 10.1128/JVI.26.2.335-343.1978.
The polycytidylic acid [poly(C)] tract in foot and mouth disease virus RNA has been located about 400 nucleotides from the 5' end of the RNA by analysis of the products from the digestion of the RNA with RNase H in the presence of oligodeoxyguanylic acid [oligo(dG)]. This treatment produces a small fragment (S) containing the small protein covalently linked to the RNA and a large fragment (L) that migrates faster than untreated RNA on low-percentage polyacrylamide gels, lacks the poly(C) tract as shown by RNase T1 digestion and oligo(dG)-cellulose binding, and is no longer infective. Polyacrylamide gel electrophoresis of fragment S suggests that it is about 400 nucleotides long, in agreement with the size estimated from the proportion of radioactivity in the fragment. Analysis of the RNase T1 digestion products of S shows that it contains only those oligonucleotides mapping close to the poly(C) tract that is situated near the 5' end of the virus RNA.
通过在寡聚脱氧鸟苷酸[oligo(dG)]存在的情况下，用核糖核酸酶H消化口蹄疫病毒RNA的产物进行分析，已确定该病毒RNA中的聚胞苷酸[poly(C)]区段位于距RNA 5'端约400个核苷酸处。这种处理产生一个小片段(S)，它含有与RNA共价连接的小蛋白，以及一个大片段(L)，该大片段在低百分比聚丙烯酰胺凝胶上的迁移速度比未处理的RNA快，经核糖核酸酶T1消化和oligo(dG)-纤维素结合显示其缺乏poly(C)区段，并且不再具有感染性。片段S的聚丙烯酰胺凝胶电泳表明它约400个核苷酸长，这与根据片段中放射性比例估计的大小一致。对S的核糖核酸酶T1消化产物的分析表明，它只包含那些位于病毒RNA 5'端附近的靠近poly(C)区段的寡核苷酸。
