Rudolf G, Wioland N, Kempf E, Bonaventure N
D.N.B.C., Centre de Neurochimie du CNRS, Strasbourg, France.
Doc Ophthalmol. 1990 Nov;76(1):47-53. doi: 10.1007/BF00140497.
The involvement of catecholaminergic and indoleaminergic systems in the modulation of the standing potential of the eye was tested in chickens by means of an indirect electrooculography method and direct current electroretinogram recordings. D,L alpha-monofluoromethyl dopa (MFMD), 50 and 100 nmol), a highly specific inactivator of aromatic L-amino acid decarboxylase, was injected intravitreally. This treatment is known to induce a selective and irreversible blockade of dopamine and 5-hydroxytryptamine biosynthesis. Five hours after drug injection an important increase in the standing potential appeared. The light peak was delayed by about 5 min but its amplitude was unchanged. The return of the standing potential to basal value during maintained illumination was delayed by 5-10 min. The time course of the dark trough was not modified. The intensity-voltage functions were also studied for the various electroretinogram components, 5 hr after MFMD. The voltage of the b-wave was reduced (by 65% with the highest intensity), while the other components were little affected. Substantial reduction in dopamine and 5-hydroxytryptamine concentrations were found in treated retinas. These data, together with previous results, suggest that the standing potential of the chicken eye may be modulated by a balance between catecholamine and indoleamine systems.
通过间接眼电图法和直流视网膜电图记录，在鸡身上测试了儿茶酚胺能和吲哚胺能系统对眼静息电位的调节作用。向玻璃体内注射50和100纳摩尔的D，Lα-单氟甲基多巴（MFMD），一种芳香族L-氨基酸脱羧酶的高度特异性灭活剂。已知这种处理会诱导多巴胺和5-羟色胺生物合成的选择性和不可逆阻断。药物注射5小时后，静息电位出现显著增加。光峰延迟约5分钟，但幅度不变。在持续光照期间，静息电位恢复到基础值的时间延迟了5-10分钟。暗谷的时间进程未改变。还研究了MFMD注射5小时后各种视网膜电图成分的强度-电压函数。b波电压降低（最高强度时降低65%），而其他成分受影响较小。在处理过的视网膜中发现多巴胺和5-羟色胺浓度大幅降低。这些数据与先前的结果一起表明，鸡眼的静息电位可能受儿茶酚胺和吲哚胺系统之间平衡的调节。
