Schütt W, Thomaneck U, Knippel E, Rychly J, Klinkmann H
Department of Internal Medicine, University Rostock, Germany.
Electrophoresis. 1990 Nov;11(11):970-5. doi: 10.1002/elps.1150111117.
The automated single cell electrophoresis microscope Parmoquant has been used for the discrimination of lymphocytes and the study of the interaction of substances with cells and synthetic particles. Electrophoretic histograms allowed the determination of changes in the proportion of lymphocyte populations after kidney transplantation, during dialysis treatment, open heart surgery and during pregnancy. Discrimination of leukemic cells on the basis of electrophoresis was used as an additional parameter in diagnosis. In a mouse tumor model, histogram determination enabled the in vivo effect of the tumor necrosis factor on immune cells to be evaluated. Cell electrophoresis was shown to be suitable to detect the influence of antibodies, lectins and bacteria on the cell surface. Protein adsorption was studied on synthetic particles using cell electrophoresis. This method was applied to investigate the phenomena of blood interaction with biomaterials used in artificial organs and to determine differences in the protein composition of serum or other body fluids connected with diseases.
自动化单细胞电泳显微镜Parmoquant已被用于淋巴细胞的鉴别以及物质与细胞和合成颗粒相互作用的研究。电泳直方图可用于确定肾移植后、透析治疗期间、心脏直视手术期间及怀孕期间淋巴细胞群体比例的变化。基于电泳对白血病细胞进行鉴别被用作诊断的一个附加参数。在小鼠肿瘤模型中，直方图测定能够评估肿瘤坏死因子对免疫细胞的体内作用。细胞电泳被证明适用于检测抗体、凝集素和细菌对细胞表面的影响。利用细胞电泳研究了合成颗粒上的蛋白质吸附。该方法被应用于研究血液与人工器官中使用的生物材料的相互作用现象，并确定与疾病相关的血清或其他体液中蛋白质组成的差异。
