Yonemura Y, Ohoyama S, Kimura H, Kamata T, Yamaguchi A, Miyazaki I
Department of Surgery II, School of Medicine, Kanazawa University, Japan.
Eur Surg Res. 1990;22(6):365-70. doi: 10.1159/000129123.
The expression of Ki-67 antigen in 71 patients with advanced gastric cancer was studied by immunohistochemical technique. Immunohistochemical staining with Ki-67 produced clear labeling of a portion of tumor cell nuclei, and the nucleoli stained intensely. The Ki-67 labeling rates of the 71 specimens ranged from 7.7 to 70.5% (mean: 29.2%; standard deviation: 12.9%). There was no significant association between Ki-67 labeling rates and macroscopic type, peritoneal metastasis, or serosal invasion. The tumors showing high Ki-67 labeling rates (greater than 25%) are more likely to have liver metastasis and lymph node involvement. Larger tumors, with a diameter greater than 6 cm, more frequently showed high Ki-67 labelling rate than those with a diameter less than 6 cm. When the Ki-67 labeling rate and 9 clinicopathologic parameters, as conventional prognostic factors, were entered simultaneously into the regression model, nodal status and Ki-67 labeling rate emerged as independent prognostic factors. These results indicate that the in situ determination of the growth fraction by Ki-67 antibody may be a reliable prognostic marker of advanced gastric cancer.
采用免疫组化技术研究了71例晚期胃癌患者Ki-67抗原的表达情况。Ki-67免疫组化染色可清晰标记部分肿瘤细胞核，核仁染色强烈。71份标本的Ki-67标记率在7.7%至70.5%之间（平均：29.2%；标准差：12.9%）。Ki-67标记率与大体类型、腹膜转移或浆膜侵犯之间无显著相关性。Ki-67标记率高（大于25%）的肿瘤更易发生肝转移和淋巴结受累。直径大于6 cm的较大肿瘤比直径小于6 cm的肿瘤更常表现出高Ki-67标记率。当将Ki-67标记率和作为传统预后因素的9个临床病理参数同时纳入回归模型时，淋巴结状态和Ki-67标记率成为独立的预后因素。这些结果表明，用Ki-67抗体原位测定生长分数可能是晚期胃癌可靠的预后标志物。
