Karangwa E, Mitchell G E, Tucker R E
Department of Animal Science, University of Kentucky, Lexington 40546-0215.
J Chromatogr. 1990 Oct 26;532(1):105-13. doi: 10.1016/s0378-4347(00)83756-1.
A method for 4-methylimidazole (4MI) extraction and quantitation in body fluids and forage samples was developed. The procedures involve ion-pair extraction of the compound with the quantitation done by ion-pair liquid chromatography. The results indicate that this high-performance liquid chromatographic method is sensitive, reproducible and more rapid than others that have been previously used. The mean recovery of 4MI from plasma and tall fescue (Festuca arundinacea) hay samples were above 95 and 85%, respectively. The versatility of the procedure makes it suitable for the determination of 4MI in body fluids and in forage samples.
开发了一种用于体液和饲料样品中4-甲基咪唑（4MI）提取和定量的方法。该程序包括用离子对萃取该化合物，并通过离子对液相色谱法定量。结果表明，这种高效液相色谱方法灵敏、可重复，且比以前使用的其他方法更快。4MI从血浆和高羊茅（Festuca arundinacea）干草样品中的平均回收率分别高于95%和85%。该程序的多功能性使其适用于体液和饲料样品中4MI的测定。
