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在根瘤菌沙漠杆菌存在的条件下,生长在铬污染土壤上的长叶相思-绒毛相思组织中的植物生长和金属分布。

Plant growth and metal distribution in tissues of Prosopis juliflora-velutina grown on chromium contaminated soil in the presence of Glomus deserticola.

机构信息

Environmental Science and Engineering, Department of Chemistry, University of Texas at El Paso, 500 West University Avenue, El Paso, Texas 79968, USA.

出版信息

Environ Sci Technol. 2010 Oct 1;44(19):7272-9. doi: 10.1021/es1008664.

Abstract

Arbuscular mycorrhizal fungi have been known to increase metal uptake in plants. In this study, mesquite (Prosopis juliflora-velutina) inoculated with Glomus deserticola or amended with EDTA were grown for 30 days in soil containing Cr(III) or Cr(VI) at 0, 40, 80, and 160 mg kg(-1). Total amylase activity (TAA) was monitored as a stress indicator. Element concentrations and distribution in tissue were determined using ICP-OES, electron scanning microprobe, and TEM. Inoculated Cr(VI) treated plants had 21% and 30% more Cr than uninoculated and EDTA treated roots, respectively, at 80 mg Cr kg(-1) treatment. In the case of Cr(III), EDTA produced the highest Cr accumulation in roots. TAA was higher in inoculated plants grown with Cr(III) at 80 and 160 mg kg(-1) and Cr(VI) at 40 and 160 mg kg(-1). The X-ray mapping showed higher metal concentrations in the vascular system of inoculated plants and the TEM micrographs demonstrated the presence of G. deserticola in roots.

摘要

丛枝菌根真菌已被证实可以增加植物对金属的吸收。在这项研究中,接种了 Glomus deserticola 的 木麻黄(Prosopis juliflora-velutina)或用 EDTA 处理的木麻黄在含有 Cr(III)或 Cr(VI)的土壤中生长 30 天,浓度分别为 0、40、80 和 160mgkg(-1)。总淀粉酶活性(TAA)被监测为应激指标。使用 ICP-OES、电子扫描探针和 TEM 确定组织中的元素浓度和分布。在 80mgCrkg(-1)处理下,接种 Cr(VI)处理的植物的 Cr 含量比未接种和 EDTA 处理的根分别多 21%和 30%。对于 Cr(III),EDTA 在根中产生了最高的 Cr 积累。在 80 和 160mgkg(-1)的 Cr(III)和 40 和 160mgkg(-1)的 Cr(VI)下,接种植物的 TAA 更高。X 射线映射显示接种植物的维管束系统中金属浓度更高,TEM 显微照片显示根中有 G. deserticola 的存在。

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