Rath Ayushee, Das Anath Bandhu
Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, Odisha 751004 India.
Centre of Excellence, North East India Studies, RUSA 2.0 Programme, New Academic Block, Utkal University, Vani Vihar, Bhubaneswar, Odisha 751004 India.
Physiol Mol Biol Plants. 2021 Feb;27(2):265-279. doi: 10.1007/s12298-021-00941-3. Epub 2021 Feb 16.
(L.) Hepper commonly known as blackgram is an important legume crop with good quality dietary proteins and vitamins. Low production of blackgram in the chromium rich soil of Odisha is a serious concern against its demand. Chromium (VI) was tested on var. B3-8-8 at 100, 150, 200, 250 and 300 µM concentration on growth, anti-oxidative enzymes and chromium content at 15, 30 and 45 d of treatments. Seed germination and growth decreased with increase dose and duration. Cr uptake induced oxidative burst with significant increase of osmolytes was observed in cell at lower doses but failed to adjust homeostasis at higher dose. Increase of GPX and SOD and decrease of CAT was observed as dose dependent. Increased protein content was detected in < 200 µM Cr concentration whereas, significant decrease of protein was noted thereafter. Down regulation of proteins (29.2 kDa and 32.6 kDa) was observed at > 250 µM of Cr. Total Cr uptake was greater in root than in shoot which might be due to poor translocation of heavy metal or detoxification. Thus, blackgram was able to maintain homeostasis at lower concentrations of Cr by activating the cascade of enzymes following cellular detoxification mechanism.
The online version of this article contains supplementary material available at (10.1007/s12298-021-00941-3).
（L.）赫珀通常被称为黑吉豆，是一种重要的豆类作物，富含优质的膳食蛋白质和维生素。在奥里萨邦富含铬的土壤中，黑吉豆产量低，无法满足需求，这是一个严重问题。以100、150、200、250和300微摩尔浓度的六价铬对品种B3 - 8 - 8进行测试，观察处理15、30和45天时对其生长、抗氧化酶和铬含量的影响。种子发芽和生长随剂量和处理时间的增加而降低。在较低剂量下，细胞中观察到铬的吸收诱导了氧化爆发，渗透溶质显著增加，但在较高剂量下无法调节体内平衡。谷胱甘肽过氧化物酶（GPX）和超氧化物歧化酶（SOD）增加，过氧化氢酶（CAT）减少，呈剂量依赖性。在铬浓度低于200微摩尔时检测到蛋白质含量增加，而此后蛋白质含量显著下降。在铬浓度高于250微摩尔时，观察到蛋白质（29.2千道尔顿和32.6千道尔顿）下调。根部的总铬吸收量大于地上部分，这可能是由于重金属转运不良或解毒作用。因此，黑吉豆能够通过细胞解毒机制激活一系列酶，在较低的铬浓度下维持体内平衡。
本文的在线版本包含补充材料，可在（10.1007/s12298 - 021 - 00941 - 3）获取。
