Department of Internal Medicine 3, Faculty of Medicine, Oita University, Yufu, Oita, Japan.
Respirology. 2010 Oct;15(7):1111-21. doi: 10.1111/j.1440-1843.2010.01825.x. Epub 2010 Aug 27.
Osteopontin is a key cytokine involved in pro-inflammatory T helper type 1 (Th1)-associated immune responses, which has recently been implicated in allergic diseases. We investigated the pathogenic role of osteopontin in eosinophilic pneumonia.
The concentrations of osteopontin and Th1- or Th2-associated cytokines were measured in BAL fluid (BALF) from 41 patients with eosinophilic pneumonia, including those with acute (AEP, n = 12), chronic (CEP, n = 16), or drug-induced eosinophilic pneumonia (DEP, n = 13). The results were compared with those from patients with other interstitial lung diseases. Immunocytochemistry and double immunofluorescence labelling were performed to determine the cellular source of osteopontin.
Osteopontin was significantly elevated in BALF from patients with eosinophilic pneumonia as compared with BALF from patients with drug-induced interstitial pneumonia, hypersensitivity pneumonitis, idiopathic interstitial pneumonia, or sarcoidosis, and also compared with BALF from healthy volunteers. Osteopontin concentrations elevated at the time of exacerbation decreased during clinical improvement, either spontaneously or as a result of corticosteroid therapy. Elevated concentrations of CXCL10, CCL17 and IL-10 were also detected in BALF from patients with eosinophilic pneumonia. Osteopontin concentrations in BALF of AEP patients were correlated with IL-5, as well as IL-10, CCL11, CCL17 and CXCL10 concentrations. In AEP and DEP patients, serum osteopontin concentrations were also elevated. Double immunofluorescence labelling showed that in patients with eosinophilic pneumonia, osteopontin was expressed in lung eosinophils.
Osteopontin is likely to contribute to the development of inflammation in patients with eosinophilic pneumonia.
骨桥蛋白是一种关键的细胞因子，参与前炎症性辅助性 T 细胞 1（Th1）相关免疫反应，最近其与过敏疾病相关。我们研究了骨桥蛋白在嗜酸性肺炎中的致病作用。
测量了 41 例嗜酸性肺炎患者（包括急性嗜酸性肺炎[AEP]患者 12 例、慢性嗜酸性肺炎[CEP]患者 16 例和药物诱导的嗜酸性肺炎[DEP]患者 13 例）支气管肺泡灌洗液（BALF）中的骨桥蛋白浓度和 Th1 或 Th2 相关细胞因子浓度。并将结果与其他间质性肺疾病患者进行了比较。进行免疫细胞化学和双免疫荧光标记以确定骨桥蛋白的细胞来源。
与药物诱导的间质性肺炎、过敏性肺炎、特发性间质性肺炎或结节病患者的 BALF 相比，以及与健康志愿者的 BALF 相比，嗜酸性肺炎患者的 BALF 中骨桥蛋白明显升高。在临床改善过程中，无论是自发性的还是皮质类固醇治疗引起的，在疾病恶化时升高的骨桥蛋白浓度均会降低。在嗜酸性肺炎患者的 BALF 中还检测到 CXCL10、CCL17 和 IL-10 浓度升高。AEP 患者的 BALF 中骨桥蛋白浓度与 IL-5 以及 IL-10、CCL11、CCL17 和 CXCL10 浓度相关。在 AEP 和 DEP 患者中，血清骨桥蛋白浓度也升高。双免疫荧光标记显示，在嗜酸性肺炎患者中，骨桥蛋白在肺嗜酸性粒细胞中表达。
骨桥蛋白可能有助于嗜酸性肺炎患者炎症的发展。
