Molecular Cancer Research Center, College of Medicine, Soonchunhyang University, Chunan, Korea.
J Exp Clin Cancer Res. 2010 Aug 27;29(1):116. doi: 10.1186/1756-9966-29-116.
Src family kinases (SFKs) play an important role in cancer proliferation, survival, motility, invasiveness, metastasis, and angiogenesis. Among the SFKs, c-Src and c-Yes are particularly over-expressed or hyper-activated in many human epithelial cancers. However, only a few studies have attempted to define the expression and role of c-Src and c-Yes in cutaneous carcinomas.
To investigate the expression of c-Src and c-Yes in cutaneous carcinomas to include malignant melanoma (MM), squamous cell carcinoma (SCC) and basal cell carcinoma (BCC).
We examined 6 normal skin tissues and 18 malignant skin tumor tissues using western blotting for the expression of c-Src and c-Yes. In another set, 16 specimens of MM, 16 SCCs and 16 BCCs were analyzed for the expression of c-Src and c-Yes using immunohistochemical staining.
Western blotting showed that c-Src was expressed in all malignant skin tumors, but not in normal skin, while c-Yes was expressed in MM and SCC, but not in BCC and normal skin. Immunohistochemical staining results of c-Src and c-Yes in MM, SCC, and BCC mirrored those of the western blot analysis.
c-Src, rather than c-Yes, plays a key role in the proliferation and progression of malignant skin cancers.
Src 家族激酶（SFKs）在癌症的增殖、存活、迁移、侵袭、转移和血管生成中发挥着重要作用。在 SFKs 中，c-Src 和 c-Yes 在许多人类上皮癌中表达过度或过度激活。然而，只有少数研究试图定义 c-Src 和 c-Yes 在皮肤癌中的表达和作用。
研究 c-Src 和 c-Yes 在皮肤癌中的表达，包括恶性黑色素瘤（MM）、鳞状细胞癌（SCC）和基底细胞癌（BCC）。
我们使用 Western blot 检测了 6 例正常皮肤组织和 18 例恶性皮肤肿瘤组织中 c-Src 和 c-Yes 的表达。在另一组中，我们使用免疫组织化学染色分析了 16 例 MM、16 例 SCC 和 16 例 BCC 中 c-Src 和 c-Yes 的表达。
Western blot 显示 c-Src 在所有恶性皮肤肿瘤中均有表达，但在正常皮肤中没有表达，而 c-Yes 仅在 MM 和 SCC 中表达，而在 BCC 和正常皮肤中没有表达。MM、SCC 和 BCC 中 c-Src 和 c-Yes 的免疫组织化学染色结果与 Western blot 分析结果一致。
c-Src 而非 c-Yes 在恶性皮肤癌的增殖和进展中发挥关键作用。
