Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.
Cell. 2010 Sep 3;142(5):714-25. doi: 10.1016/j.cell.2010.07.038.
West Nile virus (WNV) is the most common arthropod-borne flavivirus in the United States; however, the vector ligand(s) that participate in infection are not known. We now show that an Aedes aegypti C-type lectin, mosGCTL-1, is induced by WNV, interacts with WNV in a calcium-dependent manner, and facilitates infection in vivo and in vitro. A mosquito homolog of human CD45 in A. aegypti, designated mosPTP-1, recruits mosGCTL-1 to enable viral attachment to cells and to enhance viral entry. In vivo experiments show that mosGCTL-1 and mosPTP-1 function as part of the same pathway and are critical for WNV infection of mosquitoes. A similar phenomenon was also observed in Culex quinquefasciatus, a natural vector of WNV, further demonstrating that these genes participate in WNV infection. During the mosquito blood-feeding process, WNV infection was blocked in vivo with mosGCTL-1 antibodies. A molecular understanding of flaviviral-arthropod interactions may lead to strategies to control viral dissemination in nature.
西尼罗河病毒（WNV）是美国最常见的虫媒黄病毒；然而，参与感染的媒介配体尚不清楚。我们现在表明，埃及伊蚊 C 型凝集素 mosGCTL-1 被 WNV 诱导，以钙依赖性方式与 WNV 相互作用，并促进体内和体外感染。在埃及伊蚊中，一种与人 CD45 同源的蚊子蛋白 mosPTP-1，招募 mosGCTL-1 以实现病毒与细胞的附着，并增强病毒进入。体内实验表明，mosGCTL-1 和 mosPTP-1 作为同一途径的一部分发挥作用，对于 WNV 感染蚊子至关重要。WNV 的天然载体库蚊 Culex quinquefasciatus 中也观察到了类似的现象，进一步证明这些基因参与了 WNV 感染。在蚊子吸血过程中，mosGCTL-1 抗体在体内阻断了 WNV 感染。对黄病毒-节肢动物相互作用的分子理解可能会导致控制自然界中病毒传播的策略。
