Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, CA 90095-7364, USA.
Science. 2010 Aug 27;329(5995):1038-43. doi: 10.1126/science.1187433.
Construction of a complex virus may involve a hierarchy of assembly elements. Here, we report the structure of the whole human adenovirus virion at 3.6 angstroms resolution by cryo-electron microscopy (cryo-EM), revealing in situ atomic models of three minor capsid proteins (IIIa, VIII, and IX), extensions of the (penton base and hexon) major capsid proteins, and interactions within three protein-protein networks. One network is mediated by protein IIIa at the vertices, within group-of-six (GOS) tiles--a penton base and its five surrounding hexons. Another is mediated by ropes (protein IX) that lash hexons together to form group-of-nine (GON) tiles and bind GONs to GONs. The third, mediated by IIIa and VIII, binds each GOS to five surrounding GONs. Optimization of adenovirus for cancer and gene therapy could target these networks.
构建复杂的病毒可能涉及装配元件的层次结构。在这里，我们通过冷冻电镜（cryo-EM）报告了整个人腺病毒病毒粒子的 3.6 埃分辨率结构，揭示了三个次要衣壳蛋白（IIIa、VIII 和 IX）、（五邻体基底和六邻体）主要衣壳蛋白的延伸以及三个蛋白质-蛋白质网络内的原位原子模型。一个网络由顶点处的蛋白 IIIa 介导，位于六联体（GOS）瓦片内——一个五邻体基底及其周围的五个六邻体。另一个由绳索（蛋白 IX）介导，将六邻体绑在一起形成九联体（GON）瓦片，并将 GON 与 GON 结合。第三个由 IIIa 和 VIII 介导，将每个 GOS 与五个周围的 GON 结合。优化腺病毒用于癌症和基因治疗可以针对这些网络。
