Stuart David I, Oksanen Hanna M, Abrescia Nicola G A
Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
Diamond Light Source Ltd, Diamond House, Harwell Science and Innovation Campus, Didcot, UK.
Subcell Biochem. 2024;105:247-297. doi: 10.1007/978-3-031-65187-8_7.
A virus particle must work as a strongroom to protect its genome, but at the same time it must undergo dramatic conformational changes to infect the cell in order to replicate and assemble progeny. Thus, viruses are miniaturized wonders whose structural complexity requires investigation by a combination of different techniques that can tackle both static and dynamic processes. In this chapter, we will illustrate how major structural techniques such as X-ray crystallography and electron microscopy can be combined with other techniques to determine the structure of complex viruses. The power of these hybrid approaches is discussed through a number of examples.
病毒粒子必须像一个保险库一样保护其基因组，但与此同时，它必须经历剧烈的构象变化才能感染细胞，以便复制和组装后代。因此，病毒是小型化的奇迹，其结构复杂性需要通过结合不同技术来研究，这些技术能够处理静态和动态过程。在本章中，我们将说明诸如X射线晶体学和电子显微镜等主要结构技术如何能与其他技术相结合来确定复杂病毒的结构。通过多个例子讨论了这些混合方法的威力。
