School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, Korea.
J Microbiol Biotechnol. 2010 Aug;20(8):1192-5. doi: 10.4014/jmb.1004.04041.
To investigate the influence of N- or C-terminal regions of pleurocidin (Ple) peptide on the antifungal activity, four analogues partially truncated in the N- or C-terminal regions were designed and synthesized. Circular dichroism (CD) spectroscopy demonstrated that all the analogues maintained an alpha-helical structure. The antifungal susceptibility testing also showed that the analogues exhibited antifungal activities against human fungal pathogens, without hemolytic effects against human erythrocytes. The result further indicated that the analogues had discrepant antifungal activities (Ple > Ple (1-22) > Ple (4-25) > Ple (1-19) > Ple (7-25)) and that N-terminal deletion affected the activities much more than C-terminal deletion. Hydrophobicity (Ple > Ple (1-22) > Ple (4-25) > Ple (1-19) > Ple (7-25)) was thought to have been one of the consistent factors that influenced these activity patterns, rather than the other primary factors like the helicity (Ple > Ple (4-25) > Ple (1-22) > Ple (1-19) > Ple (7-25)) or the net charge (Ple = Ple (4-25) = Ple (7-25) > Ple (1-22) = Ple (1-19)) of the peptides. Taken together, the hydrophobic amino acids in the N-terminal region of Ple is more crucial for the antifungal activity than those in the C-terminal region.
为了研究 N-或 C-末端区域的抗菌肽（Ple）肽对其抗真菌活性的影响，设计并合成了四个部分在 N-或 C-末端区域被截断的类似物。圆二色性（CD）光谱表明所有类似物均保持α-螺旋结构。抗真菌药敏试验也表明，类似物对人类真菌病原体具有抗真菌活性，对人红细胞没有溶血作用。结果进一步表明，类似物具有不同的抗真菌活性（Ple > Ple（1-22）> Ple（4-25）> Ple（1-19）> Ple（7-25）），并且 N-末端缺失比 C-末端缺失对活性的影响更大。疏水性（Ple > Ple（1-22）> Ple（4-25）> Ple（1-19）> Ple（7-25））被认为是影响这些活性模式的一致因素之一，而不是其他主要因素，如螺旋性（Ple > Ple（4-25）> Ple（1-22）> Ple（1-19）> Ple（7-25））或净电荷（Ple = Ple（4-25）= Ple（7-25）> Ple（1-22）= Ple（1-19））。综上所述，Ple 的 N-末端区域的疏水性氨基酸对于抗真菌活性比 C-末端区域的更为重要。
