利用有毒病毒蛋白M2(H37A)在果蝇中进行特定细胞消融。
Specific cell ablation in Drosophila using the toxic viral protein M2(H37A).
作者信息
Lam Victoria K, Tokusumi Tsuyoshi, Cerabona Donna, Schulz Robert A
机构信息
Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
出版信息
Fly (Austin). 2010 Oct-Dec;4(4):338-43. doi: 10.4161/fly.4.4.13114. Epub 2010 Oct 1.
Abstract
The expression of toxic viral proteins for the purpose of eliminating distinct populations of cells, while leaving the rest of an organism unaffected, is a valuable method for analyzing development. Using the Gal4-UAS system, we employed the M2(H37A) toxic ion channel of the influenza-A virus to selectively ablate the Drosophila eye-antennal imaginal discs, hemocytes, dorsal vessel and nervous tissue, and comparatively monitored the effects of expressing the apoptosis-promoting protein Reaper in identical cell populations. In this report, we demonstrate the effectiveness of M2(H37A)-mediated ablation as a new means to selectively eliminate cells of interest during Drosophila development.
摘要
为了消除特定细胞群体，同时使生物体的其余部分不受影响而表达毒性病毒蛋白，是一种分析发育的有价值方法。利用Gal4-UAS系统，我们使用甲型流感病毒的M2（H37A）毒性离子通道来选择性地切除果蝇的眼触角成虫盘、血细胞、背血管和神经组织，并比较监测在相同细胞群体中表达促凋亡蛋白收割者的效果。在本报告中，我们证明了M2（H37A）介导的切除作为一种在果蝇发育过程中选择性消除感兴趣细胞的新方法的有效性。
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