Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, People's Republic of China.
Bioprocess Biosyst Eng. 2011 Feb;34(2):215-21. doi: 10.1007/s00449-010-0463-1. Epub 2010 Aug 27.
A hydrogen peroxide (H(2)O(2)) sensor was developed by electrodepositing Prussian blue (PB) on a gold electrode modified with (3-mercaptopropyl)-trimethoxysilane (MPS) polymer. The characterization of the self-assembled electrode was investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The results of electrochemical experiments showed that such constructed sensor had a favorable catalytic ability to reduce H(2)O(2). The MPS film on the modified gold electrode greatly enhanced the pH-adaptive range of PB. Large surface-to-volume ratio property of double-layer 2d-network MPS-modified PB electrode enabled stable and highly sensitive performance of the non-enzymatic H(2)O(2) sensor. The linear range of H(2)O(2) determined is from 2.0 × 10(-6) to 2.0 × 10(-4) mol L(-1) with a correlation coefficient of 0.9991 and a detection limit for H(2)O(2) of 1.8 × 10(-6) mol L(-1). The influences of the potentially interfering substances on the determination of H(2)O(2) were investigated. This modified electrode exhibits a good selectivity and high sensitivity with satisfactory results.
一种通过在金电极上电沉积普鲁士蓝（PB）来制备过氧化氢（H₂O₂）传感器的方法，该金电极先用（3-巯丙基）三甲氧基硅烷（MPS）聚合物进行了修饰。通过循环伏安法和电化学阻抗谱对自组装电极进行了表征。电化学实验结果表明，这种构建的传感器对 H₂O₂的还原具有良好的催化能力。修饰金电极上的 MPS 膜极大地增强了 PB 的 pH 适应范围。双层 2d 网络 MPS 修饰 PB 电极的大表面积与体积比特性使非酶 H₂O₂传感器具有稳定且高灵敏的性能。H₂O₂的线性范围为 2.0×10⁻⁶至 2.0×10⁻⁴mol L⁻¹，相关系数为 0.9991，H₂O₂的检测限为 1.8×10⁻⁶mol L⁻¹。研究了潜在干扰物质对 H₂O₂测定的影响。该修饰电极具有良好的选择性和高灵敏度，结果令人满意。
