Department of Medical Chemistry, Hebei Medical University, Shijiazhuang, 050017, China.
Analyst. 2010 Aug;135(8):2092-8. doi: 10.1039/c0an00019a. Epub 2010 Jun 1.
A stable and controllable Prussian blue (PB) layer electrodeposited on self-assembled monolayers (SAMs) of thioctic acid amide (T-NH(2)) on gold electrode was prepared. The PB, combining with biocompatible matrix chitosan, was used for the highly efficient immobilization of glucose oxidase (GOD) for sensitive biosensing of glucose. The PB on SAMs exhibits a good stability and a much higher catalytic ability toward the reduction of hydrogen peroxide in neutral medium, compared with that of PB directly electrodeposited on bare Au electrode. The biosensor at an applied potential of 0.0 V exhibits good anti-interferent ability, with a low detection limit of 2 microM and a much higher sensitivity (59.9 mA M(-1) cm(-2)). The apparent Michaelis-Menten constant (K(m)(app)) and apparent activation energy of enzymatic reaction were found to be 5.4 mM and 24.1 kJ mol(-1). The present method provides a promising platform for the development of new biosensors and bioelectronic devices.
在金电极上自组装硫辛酸酰胺（T-NH（2））的单层上稳定且可控地沉积普鲁士蓝（PB）层。将 PB 与生物相容性基质壳聚糖结合，用于高效固定葡萄糖氧化酶（GOD），用于葡萄糖的灵敏生物传感。与直接在裸 Au 电极上电沉积的 PB 相比，SAMs 上的 PB 对中性介质中过氧化氢的还原表现出更好的稳定性和更高的催化能力。在施加的 0.0 V 电势下，生物传感器具有良好的抗干扰能力，检测限低至 2 μM，灵敏度更高（59.9 mA M（-1）cm（-2））。酶反应的表观米氏常数（K（app））和表观活化能分别为 5.4 mM 和 24.1 kJ mol（-1）。该方法为开发新型生物传感器和生物电子器件提供了一个有前途的平台。
