College of Agriculture & Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
J Microbiol. 2010 Aug;48(4):541-5. doi: 10.1007/s12275-010-0221-9. Epub 2010 Aug 20.
To identify novel bioinsecticidal agents, a bacterial strain, Serratia sp. EML-SE1, was isolated from a dead larva of the lepidopteran diamondback moth (Plutella xylostella) collected from a cabbage field in Korea. In this study, the insecticidal activity of liquid cultures in Luria-Bertani broth (LBB) and nutrient broth (NB) of a bacterial strain, Serratia sp. EML-SE1 against thirty 3rd and 4th instar larvae of the diamondback moth was investigated on a Chinese cabbage leaf housed in a round plastic cage (Ø 10 x 6 cm). 72 h after spraying the cabbage leaf with LBB and NB cultures containing the bacterial strain, the mortalities of the larvae were determined to be 91.7% and 88.3%, respectively. In addition, the insecticidal activity on potted cabbage containing 14 leaves in a growth cage (165 x 83 x 124 cm) was found to be similar to that of the plastic cage experiment. The results of this study provided valuable information on the insecticidal activity of the liquid culture of a Serratia species against the diamondback moth.
为了寻找新型生物杀虫制剂，我们从韩国白菜地采集的菜粉蝶幼虫尸体中分离到一株细菌，命名为肠杆菌属 EML-SE1 菌株。在本研究中，我们在一个直径 10 厘米高 6 厘米的圆形塑料笼中，以白菜叶为食，检测了肠杆菌属 EML-SE1 菌株的液体培养物在 LB 肉汤（LBB）和营养肉汤（NB）中的杀虫活性，对 30 头 3 龄和 4 龄菜粉蝶幼虫进行了处理。喷洒含细菌菌株的 LBB 和 NB 培养物 72 小时后，幼虫死亡率分别为 91.7%和 88.3%。此外，在生长笼（165 x 83 x 124 cm）中含有 14 片叶子的盆栽白菜上的杀虫活性与塑料笼实验相似。本研究结果为肠杆菌属液体培养物对菜粉蝶的杀虫活性提供了有价值的信息。
