Mishra R K, Singhal J P, Datt M, Banthia A K
Department of Polymer Science, Bundelkhand University, Jhansi - India.
J Appl Biomater Biomech. 2007 May-Aug;5(2):88-94.
The design and development of pectin-based hydrogels were attempted through the chemical modification of pectin with diethanolamine (DA). Diethanolamine modified pectin (DAMP) was synthesized by the chemical modification of pectin with varying concentrations of DA (1:1,1:2,1:3 and 1:4) at 5 oC in methanol. The modified product was used for the preparation of the hydrogel with glutaraldehyde (GA) reagent. The prepared hydrogels were characterized by Fourier transform infrared (FTIR) spectroscopy; organic elemental analysis, and X-ray diffraction (XRD), and swelling, hemocompatibility and cytocompatibility studies of the prepared hydrogels were also done. FTIR spectroscopy indicated the presence of primary and secondary amide absorption bands. The XRD pattern of the DAMP hydrogel clearly indicated that there was a considerable increase in crystallinity as compared to parent pectin. The degree of amidation (DA) and molar and mass reaction yields (Ym and Yn) was calculated based on the results of organic elemental analysis. Drug release studies from the hydrogel membranes were also evaluated in a Franz's diffusion cell. The hydrogels demonstrated good water holding properties and were found to be compatible with B-16 melanoma cells and human blood.
通过用二乙醇胺（DA）对果胶进行化学改性，尝试设计和开发基于果胶的水凝胶。在5℃下于甲醇中，用不同浓度的DA（1:1、1:2、1:3和1:4）对果胶进行化学改性，合成了二乙醇胺改性果胶（DAMP）。改性产物用于与戊二醛（GA）试剂制备水凝胶。通过傅里叶变换红外（FTIR）光谱、有机元素分析和X射线衍射（XRD）对制备的水凝胶进行表征，并对制备的水凝胶进行了溶胀、血液相容性和细胞相容性研究。FTIR光谱表明存在伯酰胺和仲酰胺吸收带。DAMP水凝胶的XRD图谱清楚地表明，与母体果胶相比，结晶度有相当大的提高。根据有机元素分析结果计算了酰胺化度（DA）以及摩尔反应产率（Ym）和质量反应产率（Yn）。还在Franz扩散池中评估了水凝胶膜的药物释放研究。水凝胶表现出良好的保水性能，并且被发现与B-16黑色素瘤细胞和人血相容。
