Cruz Barrera Mauricio, Jakobs-Schoenwandt Desiree, Gómez Martha Isabel, Serrato Juan, Ruppel Silke, Patel Anant V
Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Mosquera, Km 14 Bogotá-Mosquera, Colombia.
WG Fermentation and Formulation of Biologicals and Chemicals, Faculty of Engineering and Mathematics, Bielefeld University of Applied Sciences, Bielefeld, Germany.
Biotechnol Rep (Amst). 2020 Apr 28;26:e00463. doi: 10.1016/j.btre.2020.e00463. eCollection 2020 Jun.
Despite the benefits of bacterial endophytes, recent studies on the mostly Gram-negative bacteria lack of regard for formulation strategies. The encapsulation into biopolymeric materials such as amidated pectins hydrogels is a suitable alternative. Here, this research aimed at supporting the capability of the plant growth-promoting bacteria DSM16656 to endophytically colonize plant seedlings. In this approach, the pre-conditioned cells through osmoadaptation and hydroxyectoine accumulation were used. In general, pre-osmoadapted and hydroxyectoine-supplemented bacteria cells formulated in amidated pectin dried beads increased the endophytic activity by 10-fold. Moreover, plant promotion in radish plants enhanced by 18.9% and 20.7% for a dry matter of tuber and leaves. Confocal microscopy studies with GFP-tagged bacteria revealed that bacterial aggregates formed during the activation of beads play an essential role in early colonization stages. This research encourages the integration of fermentation and formulation strategies in a bioprocess engineering approach for exploiting endophytic bacteria.
尽管细菌内生菌有诸多益处，但近期对大多革兰氏阴性菌的研究却忽视了制剂策略。将其封装到生物聚合材料中，如酰胺化果胶水凝胶，是一种合适的替代方法。在此，本研究旨在支持植物促生细菌DSM16656内生定殖于植物幼苗的能力。在该方法中，使用了通过渗透适应和羟基四氢嘧啶积累进行预处理的细胞。一般来说，用酰胺化果胶干珠配制的预渗透适应且添加了羟基四氢嘧啶的细菌细胞，其内生活性提高了10倍。此外，萝卜植株的块茎和叶片干物质产量分别提高了18.9%和20.7%。对带有绿色荧光蛋白标记细菌的共聚焦显微镜研究表明，珠子活化过程中形成的细菌聚集体在早期定殖阶段起着至关重要的作用。本研究鼓励在生物过程工程方法中整合发酵和制剂策略，以开发内生细菌。
