Dongfeng Hospital,Yunyang Medical College, Hubei, 442008, People's Republic of China.
Phytochem Anal. 2011 Jan-Feb;22(1):10-3. doi: 10.1002/pca.1243. Epub 2010 Aug 26.
Rhizoma Atractylodes Macrocephalae (RAM) contains several sesquiterpene compounds including atractylenolide III (AO-III). This bioactive compound may be used as a chemical marker for the quality control of different processed RAM products.
To develop and validate an RP-HPLC method for the quantitative determination of AO-III in RAM and in a variety of processed RAM products.
HPLC was carried out using a Kromssil C(18) RP-column eluted with methanol-water (70:30) at a flow rate of 1.0 mL/min and with UV detection at 220 nm. Full validation was performed using standard methods.
The linear range of AO-III was 5-50 µg/mL; the regression equation was y = 10210x + 11194 (r = 0.9994) and the average recovery was 101.08% (RSD = 0.98%). The detection and quantification limits for AO-III were determined to be 0.005 and 0.018 µg/mL at signal-to-noise ratios of approximately 3:1 and 10:1, respectively.
The described HPLC method is appropriate for quality assurance and differentiation of AO-III in RAM and different processed products.
苍术（RAM）含有几种倍半萜类化合物，包括苍术内酯 III（AO-III）。这种生物活性化合物可作为不同炮制苍术产品质量控制的化学标志物。
建立并验证一种反相高效液相色谱法（RP-HPLC），用于定量测定 RAM 及其多种炮制产品中的 AO-III。
采用 Kromasil C(18) RP 柱，以甲醇-水（70:30）为流动相，流速为 1.0 mL/min，在 220nm 处进行紫外检测，对 AO-III 进行 HPLC 分析。采用标准方法对方法进行全面验证。
AO-III 的线性范围为 5-50μg/mL；回归方程为 y = 10210x + 11194（r = 0.9994），平均回收率为 101.08%（RSD = 0.98%）。AO-III 的检测限和定量限分别为 0.005 和 0.018μg/mL，信噪比分别约为 3:1 和 10:1。
所描述的 HPLC 方法适用于 RAM 及其不同炮制产品中 AO-III 的质量保证和区分。
