基于 HPLC 指纹图谱、多成分定量分析及化学模式识别分析的苍术质量评价

NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China.

Molecules. 2021 Nov 25;26(23):7124. doi: 10.3390/molecules26237124.

A method for the quality evaluation of Atractylodis Macrocephalae Rhizoma (AMR) based on high-performance liquid chromatography (HPLC) fingerprint, HPLC quantification, and chemical pattern recognition analysis was developed and validated. The fingerprint similarity of the 27 batches of AMR samples was 0.887-0.999, which indicates there was very limited variance between the batches. The 27 batches of samples were divided into two categories according to cluster analysis (CA) and principal component analysis (PCA). A total of six differential components of AMR were identified in the partial least-squares discriminant analysis (PLS-DA), among which atractylenolide I, II, III, and atractylone counted 0.003-0.045%, 0.006-0.023%, 0.001-0.058%, and 0.307-1.175%, respectively. The results indicate that the quality evaluation method could be used for quality control and authentication of AMR.

建立了一种基于高效液相色谱（HPLC）指纹图谱、HPLC 定量分析和化学模式识别分析的苍术（AMR）质量评价方法，并对其进行了验证。27 批 AMR 样品的指纹相似度为 0.887-0.999，表明批次间差异很小。根据聚类分析（CA）和主成分分析（PCA），将 27 批样品分为两类。在偏最小二乘判别分析（PLS-DA）中，共鉴定出 6 种 AMR 的差异成分，其中苍术内酯 I、II、III 和苍术酮的含量分别为 0.003-0.045%、0.006-0.023%、0.001-0.058%和 0.307-1.175%。结果表明，该质量评价方法可用于 AMR 的质量控制和鉴定。