Instituto de Estructura de la Materia, CSIC, Serrano, 121, 28006-Madrid, Spain.
Langmuir. 2010 Sep 21;26(18):14663-70. doi: 10.1021/la102590f.
The adsorption of beta(2)-adrenergic agonist (βAA) drugs clenbuterol, salbutamol, and terbutaline on metal surfaces has been investigated in this work by means of surface-enhanced Raman scattering (SERS). To assist in this investigation, a previous vibrational (IR and normal Raman) characterization of these drugs was performed, supported by ab initio density functional theory calculations. The application of SERS was aimed to apply this highly sensitive technique, based on localized surface plasmon resonance, in the detection of βAA at trace concentrations and as a possible alternative method which can be postulated in routine antidoping analysis. The adsorption of these drugs was studied in depth at different experimental conditions: on Au and Ag, at different pHs, and with varying adsorbate concentration. Moreover, plasmon resonance spectroscopy was employed to investigate the adsorption of these drugs on the metal nanoparticles as well as their aggregation. It was found that the adsorption of these molecules is more effective on gold nanoparticles and at acidic pH, based on the direct interaction of the aromatic or aliphatic moieties through ionic or coordination bonds with the metal. These drugs followed a Langmuir adsorption model from which the adsorption constant and the limit of detection can be determined.
本工作采用表面增强拉曼散射（SERS）研究了β2-肾上腺素能激动剂（βAA）药物克仑特罗、沙丁胺醇和特布他林在金属表面上的吸附。为了辅助这项研究，我们对这些药物进行了之前的振动（IR 和正常拉曼）特性研究，并通过从头算密度泛函理论计算进行了支持。SERS 的应用旨在将这种基于局域表面等离激元共振的高灵敏度技术应用于痕量浓度下的βAA 检测，并作为常规反兴奋剂分析中可能假设的替代方法。我们在不同的实验条件下深入研究了这些药物的吸附：在 Au 和 Ag 上，在不同的 pH 值下，以及在不同的吸附剂浓度下。此外，等离子体共振光谱被用来研究这些药物在金属纳米粒子上的吸附及其聚集。结果发现，这些分子在金纳米粒子上和酸性 pH 值条件下的吸附更有效，这是基于芳族或脂肪族部分通过离子或配位键与金属的直接相互作用。这些药物遵循 Langmuir 吸附模型，可从中确定吸附常数和检测限。
