CellFree Sciences Co., Ltd., 75-1, Ono-cho, Leading Venture Plaza 201, Tsurumi-ku, Yokohama 230-0046, Japan.
N Biotechnol. 2011 Apr 30;28(3):211-7. doi: 10.1016/j.nbt.2010.08.009. Epub 2010 Aug 26.
Genomic information becomes useful knowledge only when the structures and functions of gene products are understood. In spite of a vast array of analytical tools developed for biological studies in recent years, producing proteins at will is still a bottleneck in post-genomic studies. The cell-free protein production system we developed using wheat embryos has enabled us to produce high quality proteins for genome-wide functional and structural analyses and at the same time circumvent almost all the limitations, such as biohazards and costs, that have hampered conventional cell-free protein synthesis systems. In the present article, we introduce examples of our new wheat germ cell-free protein production system and its application to functional and structural analyses, with the focus on the former.
只有当基因产物的结构和功能被理解时，基因组信息才能转化为有用的知识。尽管近年来为生物研究开发了大量的分析工具，但随意生产蛋白质仍然是后基因组研究的一个瓶颈。我们使用小麦胚胎开发的无细胞蛋白质生产系统使我们能够生产高质量的蛋白质，用于全基因组功能和结构分析，同时规避了常规无细胞蛋白质合成系统所面临的几乎所有限制，如生物危害和成本。在本文中，我们介绍了我们新的小麦胚无细胞蛋白质生产系统的实例及其在功能和结构分析中的应用，重点介绍前者。
