Department of Neurosurgery, Dişkapi Yildirım Beyazit Research and Education Hospital, Ankara, Turkey.
J Clin Neurosci. 2010 Dec;17(12):1548-52. doi: 10.1016/j.jocn.2010.03.043. Epub 2010 Aug 30.
The purpose of this study was to investigate the early effects of granulocyte-colony stimulating factor (G-CSF) on myeloperoxidase (MPO) activity, lipid peroxidation (LPO) and ultrastructural findings in rats after spinal cord injury (SCI). We also compared the effects of G-CSF and methylprednisolone sodium succinate (MPSS). Wistar rats were divided into four groups: control, SCI alone (50 g/cm weight drop trauma), SCI+MPSS (30 mg/kg), and SCI+G-CSF (50 μg/kg). Administration of G-CSF and MPSS significantly decreased LPO (p < 0.05) and MPO activity (p < 0.05) in the first 24 hours. MPSS was more effective than G-CSF in reducing LPO (p < 0.05) and in minimizing ultrastructure changes. The results of this study indicate that G-CSF exerts a beneficial effect by decreasing MPO activity and LPO and may reduce tissue damage in the first 24 hours after SCI. Our findings do not exclude the possibility that G-CSF has a protective effect on spinal cord ultrastructure after the first 24 hours following SCI.
本研究旨在探讨粒细胞集落刺激因子（G-CSF）对脊髓损伤（SCI）后大鼠髓过氧化物酶（MPO）活性、脂质过氧化（LPO）和超微结构的早期影响，并比较 G-CSF 和甲泼尼龙琥珀酸钠（MPSS）的作用。Wistar 大鼠分为四组：对照组、单纯 SCI 组（50 g/cm 重物坠落创伤）、SCI+MPSS 组（30 mg/kg）和 SCI+G-CSF 组（50 μg/kg）。在最初的 24 小时内，G-CSF 和 MPSS 的给药显著降低了 LPO（p<0.05）和 MPO 活性（p<0.05）。MPSS 在降低 LPO（p<0.05）和最小化超微结构变化方面比 G-CSF 更有效。本研究结果表明，G-CSF 通过降低 MPO 活性和 LPO 发挥有益作用，可能在 SCI 后最初 24 小时内减轻组织损伤。我们的研究结果并不排除 G-CSF 在 SCI 后最初 24 小时后对脊髓超微结构具有保护作用的可能性。
