一种快速微量滴定板测定法，用于测量化合物对金黄色葡萄球菌膜电位的影响。
A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential.
机构信息
Antibacterial Discovery Performance Unit, Infectious Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, PA 19426, United States.
出版信息
J Microbiol Methods. 2010 Nov;83(2):254-6. doi: 10.1016/j.mimet.2010.08.012. Epub 2010 Aug 27.
We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.
我们开发了一种基于阳离子染料 3,3'-二乙基氧羰花青碘化物（DiOC2(3)）的均相微量滴定板检测方法，用于测量化合物对金黄色葡萄球菌膜电位的影响。在对具有抗大肠杆菌活性的合成化合物库中的 372 种化合物进行筛选时，由于作用机制未知，至少有 17%的化合物表现出很强的膜活性，能够快速区分有害化合物。
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