Antibacterial Discovery Performance Unit, Infectious Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, PA 19426, United States.
J Microbiol Methods. 2010 Nov;83(2):254-6. doi: 10.1016/j.mimet.2010.08.012. Epub 2010 Aug 27.
We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.
我们开发了一种基于阳离子染料 3,3'-二乙基氧羰花青碘化物（DiOC2(3)）的均相微量滴定板检测方法，用于测量化合物对金黄色葡萄球菌膜电位的影响。在对具有抗大肠杆菌活性的合成化合物库中的 372 种化合物进行筛选时，由于作用机制未知，至少有 17%的化合物表现出很强的膜活性，能够快速区分有害化合物。
